The injection site rotation guide

By Tessaro Pty Ltd, the maker of Pepti · Reviewed

The spot you inject into matters as much as the routine you follow. Rotating sites systematically keeps tissue healthy and keeps absorption predictable.

Why rotation matters

Repeated injections into the same spot cause lipohypertrophy: fatty, rubbery or thickened tissue under the skin. Damaged tissue absorbs unpredictably — sometimes slower, sometimes faster, sometimes unevenly — so the same routine stops behaving consistently.

How to spot it

You often can't see it, but you can feel it: firm or thickened areas where you inject most. Avoid injecting into any bruised, scarred, or thickened area, and stay clear of the area immediately around the navel.

The zone system

Divide the abdomen into four numbered quadrants around the navel, then add two outer thigh zones per side. That gives eight zones in total.

Abdomen — four quadrants around the navel

1Upper left
2Upper right
3Lower left
4Lower right

Outer thighs — two zones per side

5Left thigh, upper
6Right thigh, upper
7Left thigh, lower
8Right thigh, lower

Rotate in sequence

Move through the zones in a fixed numbered order rather than by memory or comfort. Within a zone, keep each injection roughly a fingertip's width from the previous one.

Recovery

A systematic rotation gives every site the maximum possible recovery time before it's used again.

This guide describes rotation technique only. It does not recommend any substance, dose, or schedule.

If you would rather not track this by memory, Pepti, the peptide-tracking app, remembers your last site and tells you where to go next.

Not medical advice. Pepti is a record-keeping and measurement tool, not a medical device.

Get Pepti on the App Store